Abstract: Objective To investigate the relationship between Helicobacter pylori (Hp) infection and cell cycle protein D1 (CyclinD1), carbohydrate protein 72-4 (CA72-4), P53 and tumor stage in patients with gastric cancer. Methods 120 patients with gastric cancer diagnosed and treated in our hospital from June 2021 to June 2024 were divided into HP-positive group (n=30) and HP-negative group (n=30) according to their Hp infection status. Hp infection was detected by modified Giemsa staining and 13C breath test. The positive expression rates of CyclinD1 and P53 were evaluated by immunohistochemistry, and the levels of CA72-4 were detected by electrochemiluminescence immunoassay. Spearman correlation analysis was used to investigate the relationship between Hp infection and CyclinD1, CA72-4 and P53. Results The positive rates of CyclinD1, P53 and CA72-4 in HP-positive group were higher than HP-negative group, and the differences were statistically significant (P<0.05). There was no difference in tumor stage between the two groups (P>0.05). Spearman correlation analysis showed that baseline superbaseline (DOB) of Hp infection was positively correlated with CyclinD1 (r=0.781, P=0.004) and CA72-4 (r=0.553, P=0.002), but negatively correlated with P53 (r=-0.362, P=0.041). Conclusion Hp infection is closely related to the expression levels of CyclinD1, CA72-4 and P53 in gastric cancer patients, but not related to tumor stage.

Key words: helicobacter pylori, gastric cancer, Cyclin D1, Carbohydrate protein 72-4, P53, tumor staging