Auxiliary Hypolipidemic Effect and Mechanism of Laminaria Polysaccharide in ApoE-/- Mice of Atherosclerosis

Abstract

Abstract: Objective To investigate the helping hypolipidemic effect and possible mechanism of Laminaria polysaccharide in ApoE-/-mice of atherosclerosis. Methods Atherosclerotic ApoE-/-mice models were established by feeding with high-fat diet for 12 weeks and were randomly divided into model group, low-, medium- and high-dose groups and positive drug group, C57BL/6J mice fed with ordinary feed as the control group. After modeling, Laminaria polysaccharide (1 mg/kg, 2 mg/kg, 4 mg/kg) was administrated intragastrically once every other day in the treatment groups, and praluent injection (10 mg/kg) was intraperitoneally injected once a week in the positive drug group for 4 weeks. The mice in the control and model groups were simultaneously given equal volume saline. The serum levels of triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol C (LDL-C) and high-density lipoprotein cholesterol C (HDL-C) were detected by biochemical methods. Enzyme linked immunosorbent assay (ELISA) was used to determine the serum levels of leptin (LEP) and adiponectin (APN), and HE staining to observe the fat deposition in hepatic tissue. Results After the end of the administration cycle, the body weights of mice in the Laminaria polysaccharide and positive drug groups were significantly decreased than those in the model group (P<0.05). The serum levels of TG, TC and LDL-C were significantly decreased (P<0.05) while the HDL-C was significantly increased (P<0.05) than those in the model group. The serum levels of LEP and APN were significantly higher than those in the model group (P<0.05), and the fat deposition in hepatic tissue was significantly improved than model group. Conclusion Laminaria polysaccharide could improve lipid metabolism, which might related to evaluating the serum levels of LEP and APN.

Key words: Laminaria polysaccharide, atherosclerosis, lipids, leptin, adiponectin, ApoE-/-mice

CLC Number:

R285.5

XU Xin-ying, LIU Zi-shan, NI Qin-shuai, YU Zhu-qin, ZHOU Zhen. Auxiliary Hypolipidemic Effect and Mechanism of Laminaria Polysaccharide in ApoE-/- Mice of Atherosclerosis[J]. ZHONGHUA YANGSHENG BAOJIAN, 2025, 43(3): 11-15.

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