Establishment of Two Kinds of Alcohol-dependent Free Drinking Modelsin Sprague–Dawley Rats

Abstract

Abstract: Objective To establish two kinds of free drinking models of SD rats with alcohol dependence, and to provide experimental basis for animal experiment of alcohol dependence. Methods SD-rats (n=30, male) were randomly divided into control group (n=10), 10% double-bottle model group(n=10) and 20% double-bottle model group (n=10), after 28 days of modeling, the behavioral tests (elevated plus maze, morris water maze) and the scores of ethanol withdrawal syndrome were performed. Results There was no significant difference in body weight among the three groups (P>0.05), alcohol intake in the two groups reached the plateau stage, water intake decreased with the increase of alcohol intake, and alcohol preference increased to the stability (P<0.05). In the elevated plus maze test, the times of open arm entry and the retention time of open arm in the model group were significantly lower than in the control group (P<0.05), and in the morris water maze test, the memory ability in the model group was lower than in the control group (P<0.05). The 24-hour abstinence score of the model group was higher than that of the control group (P<0.05). Conclusion Both of the two methods can be used to carry out the experimental study of alcohol dependence in animals.

Key words: alcohol dependence, free drinking, elevated plus maze, morris water maze, scores of ethanol withdrawal syndrome

CLC Number:

R749

LI Qi, AN Zi-yi, DONG Ze-song, LIU Hong-feng, LI Shu, BI Peng-xiang, HUO Nan-nan, ZHU Xiao-feng. Establishment of Two Kinds of Alcohol-dependent Free Drinking Modelsin Sprague–Dawley Rats[J]. ZHONGHUA YANGSHENG BAOJIAN, 2022, 40(18): 1-4.

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