Silencing of Cathepsin B Inhibits Biological Characteristics of Papillary Thyroid Carcinoma Cells

Abstract

Abstract: Objective To explore the effect of Cathepsin B on the proliferation, migration and invasion of papillary thyroid carcinoma TPC-1 cells. Methods The siRNA of targeting Cathepsin B was transiently transfected into TPC-1 cells and Western blot was used to detect the expression of Cathepsin B. Cell counting kit-8, colony formation assay, Wound-healing migration assay and transwell assay were used to detect cell proliferation, migration and invasion. Results Western blot results showed that the expression of Cathepsin B in the experimental group was significantly lower than that in the blank group and the negative control group, and the difference was statistically significant. After silencing Cathepsin B, the ability of cell proliferation, migration, and invasion were significantly weakened, and the difference was statistically significant. Conclusion This study confirmed that silencing Cathepsin B with SiRNA significantly decreased the proliferation, migration, and invasion of TPC-1 cells, indicating that Cathepsin B may be an effective target for papillary thyroid carcinoma gene therapy.

Key words: papillary thyroid carcinoma, cathepsin B, proliferation, invasion, migration

CLC Number:

R542.22

HUANG Ting-ting, SUN Jian-hao, DU Jun-hui, LYU Yan-chun, SUN Lin-lin, ZHANG Feng-ying. Silencing of Cathepsin B Inhibits Biological Characteristics of Papillary Thyroid Carcinoma Cells[J]. ZHONGHUA YANGSHENG BAOJIAN, 2025, 43(2): 42-46.

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