ZHONGHUA YANGSHENG BAOJIAN ›› 2024, Vol. 42 ›› Issue (13): 14-18.

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Study on the Effect and Mechanism of Shenling Baizhu Powder on Intestinal Mucosal Channel Proteins in a Juvenile Rat Model of Diarrhea

MA Wei-wei1, WANG Yan-bin2, WANG Xiao-ying2, XU Guo-juan2, LIANG Cui-cai2, QI Min-min2, WEI Jia-sen3,*   

  1. 1. Pathology Teaching and Research Office, Hexi University School of Medicine, Zhangye Gansu, 734000, China;
    2. Clinical Medical College, Hexi University School of Medicine, Zhangye Gansu, 734000, China;
    3. Respiratory Department, Zhangye People's Hospital affiliated to Hexi University, Zhangye Gansu, 734000, China
  • Online:2024-07-01 Published:2024-06-25

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Abstract: Objective To explore and analysis the effects and mechanism of Shenling Baizhu Powder on intestinal mucosal channel proteins in a young mouse model of diarrhea. Methods Young rats with diarrhea (n=42) were randomly equally divided into three groups - model group, low-dose Shenling Baizhu Powder group, high-dose Shenling Baizhu Powder group. The three groups were given Shenling Baizhu Powder 0.0 g/kg(i.e. 0.9% sodium chloride solution), 1.2 g/kg, 4.6 g/kg by gavage, with the dose volume of 10 mL/kg, for 14 consecutive days. The expression of intestinal mucosal channel AQP8, CFTR in transmembrane transport protein NHE3 were detected. Results The body weight of young mice in the low-dose Shenling Baizhu Powder group and high-dose Shenling Baizhu Powder group on the 7th and 14th day of treatment were significantly higher than that in the model group (P<0.05), and the diarrhea index were significantly reduced compared to the model group (P<0.05). There were also a statistical significant difference between the high-dose Shenling Baizhu Powder group and the low-dose Shenling Baizhu Powder group (P<0.05). The ratio of urine Lactulose and Mannitol in the low-dose and high-dose Shenling Baizhu Powder groups were significantly lower than that in the model group on the 7th and 14th days of treatment (P<0.05), and the high-dose Shenling Baizhu Powder group were also lower than that in the low-dose Shenling Baizhu Powder group (P<0.05). On the 14th day of treatment, the villus length of small intestine tissue in the low-dose and high-dose Shenling Baizhu Powder groups were higher than that in the model group (P<0.05), and the crypt depth were significantly lower than that in the model group (P<0.05). There were also statistical significant difference compared between the high-dose and low-dose Shenling Baizhu Powder groups(P<0.05). On the 14th day of treatment, the relative expression levels of AQP8 protein and NHE3 protein in the small intestine tissue of the low-dose and high-dose Shenling Baizhu Powder groups were higher than those of the model group (P<0.05), while the relative expression levels of CFTR protein were lower than those of the model group (P<0.05). There were also significant difference compared between the low-dose and high-dose Shenling Baizhu Powder groups (P<0.05). Conclusion Shenling Baizhu Powder can promote the recovery of body weight, reduce the ratio of Lactulose and Mannitol in urine and diarrhea index, prolong the villus length and reduce the recess depth of small intestine, promote the expression of AQP8 protein and NHE3 protein in small intestine, and inhibit the expression of CFTR protein.

Key words: Shenling Baizhu Powder, diarrhea, mouse model, ratio of lactulose to mannitol, transmembrane transport protein sodium hydrogen exchanger 3, Aquaporin 8

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