Abstract: Objective To explore whether Yishenhuashi granules can protect diabetic nephropathy patients by activating sirt1 pathway. Methods 60 diabetic nephropathy patients admitted to Qinhuangdao First Hospital from December 2020 to February 2022 were randomly divided into experimental group and control group, with 30 patients in each group. At the same time, 20 cases of physical examination in physical examination Center of Qinhuangdao First Hospital were selected as the health examination group. The control group was given basic treatment, and the experimental group was given Yishenhuashi granule in addition to basic treatment. The total course of treatment was 3 months. Comparison of fasting blood glucose (FBG), glycosylated hemoglobin (HbA_1c), serum creatinine (Scr), uric acid (UA), blood urea nitrogen (BUN), urinary albumin excretion rate (UAER), estimated glomerular filtration rate (eGFR), β2 microglobulin (β2-MG) and silent information regulator 1(sirt1) level. Results There was no statistical difference between the healthy control group,The test group and the control group. Before treatment, there were no significant differences in FBG, HbA_1c, Scr, UA, BUN, UAER, eGFR, β2-MG and sirt1 between the experimental group and the control group (P>0.05). After treatment, Scr levels in the experimental group decreased significantly compared with the control group, with statistical significance (P<0.05). There was no statistical significance in other laboratory indexes between groups (P>0.05). SIRT1 level in diabetic nephropathy patients was significantly lower than that in health examination group, with statistical significance (P<0.05). Conclusion ①Yishenhuashi granules can significantly reduce Scr value and improve renal function. ②sirt1 may be a protective factor in diabetic nephropathy. ③It is not concluded that Yishenhuashi granules can protect diabetic nephropathy patients by activating sirt1 pathway.

Key words: yishenhuashi granules, diabetic nephropathy, silent information regulator 1