Abstract: Objective Newborns with suspected neonatal hemolytic disease are screened for three hemolytic diseases. To understand the distribution of HDN detection rate in the blood type, gender, birth date age and number of pregnancies of the mother. Methods Blood samples from 402 suspected ABO-HDN newborns and their mothers were collected from our hospital. Both specimens were identified for blood type and the mother was screened for irregular antibodies. Neonatal specimens were tested for hemolytic blood (direct anti-human globulin test, free antibody test, and release test) and analyzed. Results There were 222 accounting for 55.22% confirmed cases of the disease in 402 neonates with suspected ABO-HDN. The positive rates of ABO-HDN in newborns with blood type A and blood type B were 62.86% and 49.34%, respectively. The positive rates of ABO-HDN in male and female newborns were 48.06% and 63.73%, respectively. The positive rate of newborns with a birth age of≤1 day was 77.23%. The positive rate of newborns at 2~3 days was 57.46%; the positive rate of newborns >3 days was 33.33%. The positive rates of ABO-HDN in mothers with first pregnancy and non-first pregnancy were 47.40% and 61.14%, respectively. Conclusion The positive detection rate of ABO-HDN was different in children with different blood types and genders. The positive detection rate of ABO-HDN was also different according to the age of birth and the number of pregnancies.

Key words: ABO-HDN, direct antibiosis test, free antibody test, dispersion test