Abstract: Objective To investigate the role and transcriptional level of histone deacetyltransferase Sirt1 in liver regeneration after hepatectomy. Methods Three mice were randomly selected and executed at 12 h, 24 h, 48 h and 72 h postoperatively in the experimental group, and the mice in control group were executed immediately after the sham operation.The ability of liver regeneration was assessd by liver weight/body weight ratio, the degree of liver injury was detected by ALT activity detection and the transcriptional level of histone deacetyltransferase Sirt1 in mouse liver tissue was detected by real-time quantitative PCR (RT-qPCR). Results Aftter hepatectomy, the liver weight/body weight ratio in the experimental group increased gradually. compared with the control group, ALT levels in serum were higher at 12 h, 24 h and 48 h, and recovered at 72 h. At 48 h, the transcriptional level of histone deacetyltransferase Sirt1 decreased. Conclusion Liver regeneration after hepatectomy in mice might be related to the decrease of histone deacetyltransferase Sirt1 transcription, which resulted in the increase of acetylation of some proteins.

Key words: hepatectomy, acetylating, liver regeneration