Abstract: Objective By analyzing the blood drug concentrations of patients with fungal infections treated with voriconazole and fluconazole alone, we aim to explore the clinical significance of serum drug concentrations in patients' antifungal treatment and promote individualized and precise drug therapy. Methods Blood samples from patients with fungal infections treated with oral or intravenous voriconazole and fluconazole from May 2022 to December 2023 were collected and their blood drug concentrations were detected. Among them, there were 360 blood samples from patients with fungal infections treated with voriconazole alone and 109 blood samples from patients with fungal infections treated with fluconazole alone. Homogeneous enzyme immunoassay (HEIA) was used to detect the serum drug concentrations of voriconazole and fluconazole, and the results were statistically analyzed. Results 469 samples were collected for testing. Among them, 360 blood samples from patients with fungal infections treated with voriconazole alone involved 170 patients. The number of patients with fungal infections treated with voriconazole alone who underwent blood drug concentration testing was relatively high; 109 blood samples from patients with fungal infections treated with fluconazole alone involved 86 patients. The number of patients with fungal infections treated with fluconazole alone who underwent blood drug concentration testing was relatively low; the serum drug concentration of voriconazole was controlled within the effective concentration range; the serum drug concentration of fluconazole was controlled within the effective concentration range; some patients using voriconazole and fluconazole had interactions with other drugs. Conclusion After the application of antifungal drugs in patients with fungal infections, the detection of serum drug concentrations is crucial for guiding the next step of clinical treatment of antifungal infections. It is recommended to adjust the drug regimen according to the serum drug concentrations of patients after medication in a timely manner, and develop individualized drug regimens to ensure the safety, effectiveness, and economy of drug treatment for patients.

Key words: drug concentration detection, serum drug concentration, homogeneous enzyme amplification immunoassay