Abstract: Objective Exploring the effect of nutritional supplements on improving endurance running ability in handball players with low hemoglobin levels. Methods Twelve female handball players from Anhui Province who participated in the preliminary round of the 14th National Games were selected. Five athletes whose endurance running test results were lower than the threshold standard of the National Games were selected as the observation group, and the other seven athletes were selected as the control group. Both the control group and the observation group were trained according to the pre competition training plan, and their diet was managed uniformly. They ate three meals a day at the athletes’ restaurant as usual.The observation group added ass hide glue, folic acid, Vitamins With Minerals Tablets(29), ferrous sulfate and other nutritional supplements for comprehensive intervention, and the intervention training was 8 weeks. Compare the biochemical indicators of hemoglobin, serum ferritin, blood urea nitrogen, serum creatine kinase, serum testosterone, cortisol and physical fitness indicators of endurance running performance in athletes before and after intervention. Results After 8 weeks of intervention, the endurance running performance of the experimental group improved （P<0.05), and compared with the control group, the improvement in performance was higher （P<0.05); The hemoglobin level in the experimental group increased （P<0.05), and the increase was higher than that in the control group; The serum transferrin level in the experimental group increased （P<0.05), and the increase was higher than that in the control group （P<0.05). Conclusion Drug intervention combined with lactate tolerance training can effectively improve the endurance running ability and hemoglobin level of female athletes.

Key words: nutritional supplements, female athletes, endurance running, hemoglobin, serum ferritin