新生儿溶血性黄疸的肠道菌群结构特点分析

摘要/Abstract

摘要： 目的明确新生儿溶血性黄疸的肠道菌群结构和变化特点。方法选取2022年10月—2023年3月于徐州医科大学附属宿迁医院出生且确诊为新生儿溶血性黄疸的新生儿作为病例组,同期于医院出生的健康新生儿作为对照组。提取所有新生儿的粪便基因组DNA并进行质量检测,合格后进行16S rDNA扩增子测序。随后对测序获取的生物信息进行多样性、丰度和结构分析。结果 α多样性分析显示病例组的菌群多样性增加,但丰度未见明显改变。β多样性分析显示两组间的肠道菌群相似性存在差异。LEfSe分析显示病例组肠道菌群结构发生改变,主要表现为埃希氏菌属、克雷伯菌属、拟杆菌属和罗氏菌属丰度升高,而链球菌属丰度降低。结论溶血性黄疸新生儿存在肠道菌群紊乱,以条件致病菌属丰度升高为主要特征。该试验结果可能为新生儿溶血性黄疸的靶向微生态干预提供部分理论依据。

关键词: 新生儿溶血病, 黄疸, 新生儿, 肠道菌群

Abstract: Objective To clarify the structural characteristics and changes of intestinal microbiota in neonatal hemolytic jaundice. Methods Newborns born in Suqian Hospital Affiliated to Xuzhou Medical University from October 2022 to March 2023 and diagnosed with neonatal hemolytic jaundice were selected as the case group. Healthy newborns born at the same time in the hospital served as the control group. We extracted the fecal genomic DNA of all newborns and conducted quality testing. After passing the test, 16S rDNA amplicon sequencing was performed. Subsequently, diversity, abundance, and structural analysis were conducted on the biological information obtained through sequencing. Results α Diversity analysis showed an increase in microbial diversity in the case group, but there was no significant change in abundance. β Diversity analysis showed differences in gut microbiota similarity between the two groups. LEfSe analysis showed changes in the gut microbiota structure of the case group, mainly manifested as an increase in the abundance of Escherichia, Klebsiella, Bacteroides, and Rothia, while a decrease in the abundance of Streptococcus. Conclusion Neonates with hemolytic jaundice have intestinal microbiota disorder, mainly characterized by an increase in the abundance of conditional pathogenic bacteria. The results may provide some theoretical basis for targeted microecological intervention in neonatal hemolytic jaundice.

Key words: neonatal hemolytic disease, jaundice, newborns, intestinal microbiota

中图分类号:

R722.1

闫怀宇, 乔继冰, 蒋亚洲, 沈男, 吴让, 宁涛. 新生儿溶血性黄疸的肠道菌群结构特点分析[J]. 中华养生保健, 2024, 42(4): 4-8.

YAN Huai-yu, QIAO Ji-bing, JIANG Ya-zhou, SHEN Nan, WU Rang, NING Tao. Analysis of the Structural Characteristics of Intestinal Microbiota in Newborns with Hemolytic Jaundice[J]. ZHONGHUA YANGSHENG BAOJIAN, 2024, 42(4): 4-8.

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