TLR4/NF-κB p65高表达对皮肤鳞状细胞癌增殖、侵袭和转移的影响

摘要/Abstract

摘要： 目的探讨与分析Toll样受体4（Toll-like recepTor 4,TLR4）/核因子KappaB（NF-κB）p65高表达对皮肤鳞状细胞癌增殖、侵袭和转移的影响。方法对数生长期的人皮肤鳞状细胞癌A431细胞株分为三组,空白组、对照组与实验组,分别转染DMEM培养基、pc3.0空载体、pc3.0-TLR4NF-κB p65载体,检测皮肤鳞状细胞癌增殖、侵袭和转移表达情况。结果转染24 h、48 h后,实验组的TLR4、NF-κB p65 RNA表达水平显著高于空白组与对照组,差异有统计学意义（P<0.05）,空白组与对照组比较,差异无统计学意义（P>0.05）。实验组的细胞增殖指数显著低于空白组与对照组,差异有统计学意义（P<0.05）,对照组与空白组比较,差异无统计学意义（P>0.05）。实验组的细胞侵袭指数与细胞转移指数都明显低于空白组与对照组,差异有统计学意义（P<0.05）,对照组与空白组比较,差异无统计学意义（P>0.05）。实验组的Caspase-9/Myc蛋白表达水平显著高于空白组与对照组,差异有统计学意义（P<0.05）,空白组与对照组比较,差异无统计学意义（P>0.05）。结论 TLR4/NF-κB p65高表达能抑制皮肤鳞状细胞癌细胞的增殖、转移与侵袭,也可促进Caspase-9/Myc蛋白的表达,从而发挥促进肿瘤细胞凋亡的作用。

关键词: Toll样受体4, 核因子KappaB p65, 皮肤鳞状细胞癌细胞, 细胞增殖, 细胞侵袭, 细胞凋亡

Abstract: Objective To explore and analysis the effects of Toll like receptor 4 (TLR4)/nuclear factor NF-κBp65 high expression on the proliferation, invasion, and metastasis of skin squamous cell carcinoma. Methods Human skin squamous cell carcinoma A431 cell line with logarithmic growth phase were divided into three groups - blank group, control group, and experimental group. They were transfected with DMEM medium, pc3.0 empty vector, and pc3.0-NF-κBp65, respectively vector. All the cells were used to detect the expression of proliferation, invasion, and metastasis in skin squamous cell carcinoma. Results After 24 and 48 hours of transfection, the expression of TLR4 and NF-κBp65 in the experimental group were significantly higher than that of the blank group and control group (P<0.05), and there were no significant difference compared between the blank group and control group (P>0.05). The cell proliferation index of the experimental group were significantly lower than that of the blank group and control group (P<0.05), and there were no significant difference compared between the control group and the blank group (P>0.05). The cell invasion index and cell metastasis index of the experimental group were significantly lower than those of the blank group and control group (P<0.05), while there were no significant difference compared between the control group and the blank group (P>0.05). The expression level of Caspase-9/Myc protein in the experimental group were significantly higher that of the blank group and control group (P<0.05), and there were no significant difference compared between the blank group and control group (P>0.05). Conclusion TLR4/NF- κBp65 high expression can inhibit the proliferation, metastasis, and invasion of skin squamous cell carcinoma cells, as well as promote the expression of Caspase-9/Myc protein, thereby exerting promote apoptotic effect on tumor cells.

Key words: toll like receptor 4, nuclear factor KappaB p65, skin squamous cell carcinoma cells, cell proliferation, cell invasion, cell apoptosis

中图分类号:

刘莉. TLR4/NF-κB p65高表达对皮肤鳞状细胞癌增殖、侵袭和转移的影响[J]. 中华养生保健, 2024, 42(8): 37-40.

LIU Li. TLR4/NF- κBp65 the Effect of High Expression of on the Proliferation, Invasion, and Metastasis of Skin Squamous Cell Carcinoma[J]. ZHONGHUA YANGSHENG BAOJIAN, 2024, 42(8): 37-40.

参考文献

[1] 李文静,肖钟,王新,等.沉默circ_0000218靶向miR-140-3p影响皮肤鳞状细胞癌细胞恶性生物学行为[J].中国中西医结合皮肤性病学杂志,2023,22(1):1-7.
[2] 谢立伟,杨浩,杨洁.miR-338-3p调控头颈部鳞状细胞癌自噬、增殖、凋亡的作用及其机制[J].现代医学,2022,50(3):273-279.
[3] 张洋,吕君,陈尚周,等.槲皮黄酮抑制LRIG2/EGFR信号转导诱导人皮肤鳞状细胞癌细胞凋亡[J].中国中西医结合皮肤性病学杂志,2022,21(1):1-5.
[4] 王佳雯,朱君.伊立替康活性代谢物SN38对皮肤鳞状细胞癌细胞坏死性凋亡的调节机制[J].浙江临床医学,2022,24(6):814-817.
[5] 吴敏,李丽,陈鹏飞,等.萝卜硫素通过ANXA3/p38和JNK信号通路诱导人皮肤鳞状细胞癌细胞凋亡[J].中国中西医结合皮肤性病学杂志,2022,21(5):398-403.
[6] 丁蕾,白慧英,严玉月.红花多糖通过circ_0014130/miR-127-5p对皮肤鳞状细胞癌细胞的影响[J].中国免疫学杂志,2022,38(3):353-357.
[7] 鲁青莲,刘林莉,蒋亚辉,等.红芪多糖通过线粒体途径诱导皮肤鳞状细胞癌细胞SCL-1凋亡的研究[J].河北医学,2022,28(2):208-213.
[8] 周昕,牛恺文,邓泽义.FGFR1对头颈部鳞状细胞癌细胞增殖和凋亡影响机制研究[J].中华肿瘤防治杂志,2022,29(9):635-643.
[9] 周钰,黎晓红,袁雪萍,等.circ_0091581靶向miR-136对皮肤鳞状细胞癌细胞生物学行为的影响[J].医学研究生学报,2022,35(12):1274-1278.
[10] 郭金兰,甘才斌,张洁,等.miR-17-5p靶向PI3K/AKT信号通路调控皮肤鳞状细胞癌细胞增殖、凋亡的机制[J].中国老年学杂志,2021,41(12):2599-2603.
[11] 张翠,王珺,林伟,等.LINC00460靶向miR-380-3p对皮肤鳞状细胞癌细胞增殖、迁移、侵袭和凋亡的影响[J].现代肿瘤医学,2021,29(24):4275-4281.
[12] 郜亮,贾振宇.miR-194-5p靶向TSPAN1基因对皮肤鳞状细胞癌细胞增殖、凋亡及侵袭的影响[J].中国免疫学杂志,2021,37(18):2245-2251.
[13] 李晓民,梁韦巍,韩冬,等.葡萄籽原花青素对人皮肤鳞状细胞癌A431细胞增殖和凋亡的影响[J].中国麻风皮肤病杂志,2021,37(11):696-699.
[14] 王莉,陈尚周,雷淑英,等.跨膜受体蛋白NOTCH1诱导人皮肤鳞状细胞癌细胞凋亡的机制研究[J].中国中西医结合皮肤性病学杂志,2021,20(6):577-581.
[15] 张艳靖,朱青青,周海霞,等.环状RNA BICD2对口腔鳞状细胞癌细胞生物学行为的影响及机制研究[J].中华口腔医学杂志,2021,56(11):1098-1108.
[16] 李旭阳,尹光文,郑云鹏,等.circ_0007059/miR-421对皮肤鳞状细胞癌发生及发展的影响[J].中国皮肤性病学杂志,2021,35(11):1216-1225.
[17] 刘彩玉,杨丽亚,田冬梅.萝卜硫素通过lncRNA MALAT1/TFEB信号通路诱导人皮肤鳞状细胞癌细胞凋亡机制探讨[J].中国美容医学,2021,30(2):99-102.
[18] 任永强,李庆华,黄新建,等.PTTG抑制PI3K/AKT信号通路诱导皮肤鳞状细胞癌细胞凋亡的机制[J].临床与实验病理学杂志,2020,36(1):92-95.
[19] 林简,刘晓红,王荣坤,等.干扰NDRG1表达通过调控PI3K/Akt信号通路对皮肤鳞状细胞癌细胞凋亡的影响[J].中国皮肤性病学杂志,2020,34(9):1005-1011.
[20] 刘鸿雁,孙冲,李倩,等.氟伐他汀钠对皮肤鳞状细胞癌细胞增殖、凋亡及PI3K/AKT/mTOR通路的影响[J].中国医科大学学报,2020,49(9):841-845.