脑蛋白水解物干预VaD小鼠海马神经元凋亡及可能的机制

中华养生保健 ›› 2024, Vol. 42 ›› Issue (2): 4-5.

脑蛋白水解物干预VaD小鼠海马神经元凋亡及可能的机制

王素平¹, 武筱林², 王帅¹, 宋涛¹, 庄洁¹, 兰小磊^3,*

1.青岛市第三人民医院神经康复科,山东青岛,266041;
2.青岛大学医学部中西医结合中心,山东青岛,266021;
3.青岛大学附属医院神经外科,山东青岛,266003

出版日期:2024-01-16 发布日期:2024-01-17
通讯作者: ^*兰小磊,E-mail：18661809183@163.com。
作者简介:王素平（1971—）,女,汉族,籍贯：山东省青岛市,硕士研究生,主任医师,研究方向：神经康复医学。
基金资助:
青岛市医药卫生科研指导项目（2022-WJZD104）

The Interferingeffect and Possible Mechanism of Cerebroprotein Hydrolysate-Ionthe Apoptosis of Hippocampal Neurons in Mice with Vascular Dementia

WANG Su-ping¹, WU Xiao-lin², WANG Shuai¹, SONG Tao¹, ZHUANG jie¹, LAN Xiao-lei^3,*

1. Department of Neuro-rehabilitation, Qingdao Third People's Hospital, Qingdao Shangdong 266041, China;
2. Institute of Integrative Medicine, Qingdao University Medical College, Qingdao Shandong 266021, China;
3. Department of Neurosurgery, Affiliated Hospital of Qingdao University, Qingdao Shandong 266003, China

Online:2024-01-16 Published:2024-01-17

摘要/Abstract

摘要： 目的本研究旨在探讨脑蛋白水解物-I（CH-I）干预血管性痴呆（VaD）小鼠海马神经元凋亡的作用及可能的机制。方法通过Y迷宫从70只雄性昆明小鼠中筛选出学习能力较好的小鼠60只,随机选择10只为假手术组,其余50只建立VaD小鼠模型,随机分为模型组,脑活素阳性药组,CH-I低、中、高剂量组[10、20、30 mg/（kg·d）体质量],连续给药4周。末次给药后,应用Y迷宫检测小鼠的学习记忆能力,采用HE染色观察小鼠海马区细胞的形态结构,TUNEL检测小鼠海马组织细胞凋亡,Western blot分析海马组织Akt、p-Akt、Bcl-2、Caspase-9（Casp-9）、Caspase-3（Casp-3）表达。结果脑蛋白水解物-I可改善VaD小鼠学习记忆能力,改善海马神经细胞的形态结构和凋亡,增强p-Akt、Bcl-2表达,同时抑制Casp-9和Casp-3表达。结论 CH-I可能通过调节PI3K/Akt信号通路而抑制VaD小鼠海马神经细胞凋亡。

关键词: 脑蛋白水解物-I, VaD, 海马, 凋亡, PI3K/Akt, 小鼠

Abstract: Objective This paper aims to explore the interfering effect and and possible mechanismof Cerebroprotein Hydrolysate-I (CH-I) on hippocampal neurons in mice of vascular dementia (VaD). Methods 60 male KM micewith good learning ability were selected fromtotal of 70 male KM micethrough the Y maze. Then, 10 mice were randomly selected as the sham operation group, and the remaining 50 mice were used to establish VaD mouse model. The successful modeled mice were randomly divided into model group, Cerebrolysin (CBL) positive drug, low-dose, medium-dose, and high-dose groups received CH-I intraperitoneal injection (10, 20, 30 mg/kg body weight) dailyfor continuous administration 4 weeks. After the last administration, the learning and memory ability of mice were measured using Y maze. The cell morphology and structure of hippocampal cellswere observed by HE staining andthe apoptosis detected by TUNEL to detect, Western blot was used to analysis he protein expressions of p-Akt, Bcl-2, Caspase-9 (Casp-9), and Caspase-3 (Casp-3) in hippocampus. Results CH-I can improve the learning and memory ability of VaD mice, improve the shape and structure and apoptosis of neurons in the hippocampus, enhance the expressions of p-Akt and Bcl-2, and simutanouslyinhibit Casp-9 and Casp-3 expressions. Conclusion CH-I might inhibit apoptosisof hippocampal neurons in VaD mice byregulatingthe PI3K/Akt signaling pathway.

Key words: Cerebroprotein Hydrolysate-I, VaD, hippocampus, apoptosis, PI3K/Akt, mice

中图分类号:

R741.05

王素平, 武筱林, 王帅, 宋涛, 庄洁, 兰小磊. 脑蛋白水解物干预VaD小鼠海马神经元凋亡及可能的机制[J]. 中华养生保健, 2024, 42(2): 4-5.

WANG Su-ping, WU Xiao-lin, WANG Shuai, SONG Tao, ZHUANG jie, LAN Xiao-lei. The Interferingeffect and Possible Mechanism of Cerebroprotein Hydrolysate-Ionthe Apoptosis of Hippocampal Neurons in Mice with Vascular Dementia[J]. ZHONGHUA YANGSHENG BAOJIAN, 2024, 42(2): 4-5.

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