IRF9、PSMA5联合外周血巨噬细胞M1/M2极化状态预测类风湿性关节炎进展的多元回归模型

中华养生保健 ›› 2024, Vol. 42 ›› Issue (19): 4-8.

IRF9、PSMA5联合外周血巨噬细胞M1/M2极化状态预测类风湿性关节炎进展的多元回归模型

关岳¹, 李新¹, 杨贺旻², 徐思宇³, 史丽东¹, 刘扬洋¹, 孔令丹¹, 秦影^1,*

1.黑龙江省齐齐哈尔医学院附属第三医院风湿免疫科,黑龙江齐齐哈尔,161006;
2.黑龙江省齐齐哈尔医学院附属第三医院中心实验室,黑龙江齐齐哈尔,161006;
3.黑龙江省齐齐哈尔医学院附属第三医院检验中心,黑龙江齐齐哈尔,161006

出版日期:2024-10-01 发布日期:2024-09-25
通讯作者: ^*秦影,E-mail：Qy15603629068@qmu.edu.cn。
作者简介:关岳（1987—）,女,满族,籍贯：黑龙江省绥化市,硕士研究生,主治医师,研究方向：风湿免疫。
基金资助:
2023年黑龙江省省属本科高校基本科研业务费项目（2023-KYYWF-0878）

Multiple Regression Model of IRF9, PSMA5 Combined with M1/M2 Polarization Status of Peripheral Blood Macrophages to Predict Rheumatoid Arthritis Progression

GUAN Yue¹, LI Xin¹, YANG He-min², XU Si-yu³, SHI Li-dong¹, LIU Yang-yang¹, KONG Ling-dan¹, QIN Ying^1,*

1. Department of rheumatology and immunology, the Third Affiliated Hospital of Qiqihar Medical University, Qiqihar Heilongjiang, 161006, China;
2. Central Laboratory, the Third Affiliated Hospital of Qiqihar Medical University, Qiqihar Heilongjiang, 161006, China;
3. Inspection Center, the Third Affiliated Hospital of Qiqihar Medical University, Qiqihar Heilongjiang, 161006, China

Online:2024-10-01 Published:2024-09-25

摘要/Abstract

摘要： 目的探究干扰素调节因子9（IRF9）、蛋白酶体a5亚基（PSMA5）联合外周血巨噬细胞经典激活（M1）和替代激活（M2）极化状态对类风湿性关节炎（RA）进展的预测价值。方法选取2022年2月—2023年2月齐齐哈尔医学院附属第三医院收治的87例RA患者,根据疾病严重程度将其分为活动期组和缓解期组。比较两组患者IRF9、PSMA5、M1/M2比值,采用二元Logistic回归分析其与RA进展的关系,构建风险预测模型,利用ROC曲线、校准曲线与决策曲线评价其预测价值。结果缓解期组共49例患者（56.32%）,活动期组共38例患者（43.68%）,两组在累及关节数、关节压痛指数及关节肿胀指数等方面,差异有统计学意义（P<0.05）;活动期患者IRF9、PSMA5、M1、M1/M2比例显著高于缓解期组,但M2显著低于缓解期组,差异有统计学意义（P<0.05）; Logistic回归分析显示IRF9、PSMA5、M1/M2比值是RA疾病进展的危险因素;ROC分析用于预测RA进展,AUC值显示M1/M2为0.690,IRF9为0.664,PSMA5为0.733。结论 M1/M2、IRF9、PSMA5与RA疾病进展相关,有助于评估RA疾病进展。

关键词: 类风湿关节炎, 巨噬细胞, 干扰素调节因子9, 蛋白酶体a5亚基

Abstract: Objective To study the predictive value of IRF9 and PSMA5 combined with M1 and M2 macrophage polarization on rheumatoid arthritis (RA) progression. Methods A total of 87 RA patients were divided into active and remission groups based on disease severity. IRF9 and PSMA5 expression, M1 and M2 macrophage proportions, and the M1/M2 ratio were compared. Logistic regression and ROC analysis were used to assess predictive performance. Results Higher expression of IRF9 and PSMA5 was seen in active RA patients. M1/M2 ratio was higher in active RA. Logistic regression identified IRF9, PSMA5, and M1/M2 ratio as RA progression risk factors. AUC values for predicting RA progression were 0.690 for M1/M2, 0.664 for IRF9, and 0.733 for PSMA5. Conclusion M1/M2 polarization, IRF9, and PSMA5 levels were elevated in RA patients and correlated with disease progression. These markers could be valuable for assessing and evaluating RA progression clinically.

Key words: rheumatoid arthritis, macrophage, interferon regulatory factor 9, proteasome a5 subunit

中图分类号:

R593.22

关岳, 李新, 杨贺旻, 徐思宇, 史丽东, 刘扬洋, 孔令丹, 秦影. IRF9、PSMA5联合外周血巨噬细胞M1/M2极化状态预测类风湿性关节炎进展的多元回归模型[J]. 中华养生保健, 2024, 42(19): 4-8.

GUAN Yue, LI Xin, YANG He-min, XU Si-yu, SHI Li-dong, LIU Yang-yang, KONG Ling-dan, QIN Ying. Multiple Regression Model of IRF9, PSMA5 Combined with M1/M2 Polarization Status of Peripheral Blood Macrophages to Predict Rheumatoid Arthritis Progression[J]. ZHONGHUA YANGSHENG BAOJIAN, 2024, 42(19): 4-8.

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