上调miR-590-3p对胰腺癌细胞侵袭转移的作用及影响机制

摘要/Abstract

摘要： 目的观察miR-590-3p基因表达对人胰腺癌Capan-2细胞侵袭转移能力的影响。方法人胰腺癌Capan-2细胞分为两组：阴性对照组（NC组）及转染miR-590-3p mimics组（miR-590-3p mimics组）。采用qRT-PCR法检测正常胰腺导管上皮细胞hTERT-HPNE以及胰腺癌Capan-2细胞中miR-590-3p的表达,通过流式细胞术检测miR-590-3p对Capan-2细胞凋亡的影响,通过MTT实验检测miR-590-3p对Capan-2细胞增殖的影响,通过划痕实验检测细胞迁移能力的变化,通过Transwell实验检测细胞侵袭能力的变化,采用Western blot法检测AKT/mTOR通路相关蛋白表达变化。结果胰腺癌Capan-2细胞较正常胰腺导管上皮细胞中miR-590-3p表达水平显著降低,差异有统计学意义（P<0.05）;与NC组相比,miR-590-3p mimics组miR-590-3p mRNA表达水平显著升高,差异有统计学意义（P<0.05）;流式细胞术实验结果显示,与NC组相比,miR-590-3p mimics组细胞凋亡水平显著升高,差异有统计学意义（P<0.05）;细胞划痕及侵袭实验结果显示,与NC组相比,miR-590-3p mimics组细胞迁移和侵袭能力显著降低,差异有统计学意义（P<0.05）;Western blot结果显示,与NC组相比,miR-590-3p mimics组中p-AKT和p-mTOR蛋白水平明显降低,差异有统计学意义（P<0.05）。结论上调miR-590-3p表达可增加胰腺癌细胞凋亡能力,抑制胰腺癌细胞迁移和侵袭能力。

关键词: 胰腺癌, miR-590-3p, 侵袭, 转移

Abstract: Objective To observe the effect of miR-590-3p gene expression on the invasion and metastasis of human pancreatic cancer Capan-2 cells. Methods Human pancreatic cancer Capan-2 cells were divided into two groups: negative control group (NC group) and miR-590-3p up-regulated group (miR-590-3p mimics group). The expression of miR-590-3p in human normal pancreatic ductal epithelial cells hTERT-HPNE and pancreatic cancer Capan-2 cells was detected by qRT PCR. The effect of miR-590-3p on the apoptosis of Capan-2 cells was detected by flow cytometry, the effect of miR-590-3p on the proliferation of Capan-2 cells was detected by MTT test, the change of cell migration ability was detected by scratch test, and the change of cell invasion ability was detected by Transwell test, the expression of AKT/mTOR pathway related proteins was detected by Western blot. Results The expression level of miR-590-3p in Capan-2 cells of pancreatic cancer was significantly lower than that in normal pancreatic duct epithelial cells, and the expression level of miR-590-3p mRNA in miR-590-3p mimics group was significantly higher than that in NC group (P<0.05). The results of flow cytometry showed that the level of apoptosis in miR-590-3p mimics group was significantly higher than that in NC group (P<0.05). The results of cell scratch and invasion test showed that the migration and invasion ability of miR-590-3p mimics group was significantly lower than that of NC group (P<0.05). Western blot results showed that compared with the NC group, the levels of p-AKT and p-mTOR proteins in the miR-590-3p mimics group were significantly reduced. Conclusion Up regulation of miR-590-3p expression can increase the apoptosis of pancreatic cancer cells and inhibit the migration and invasion of pancreatic cancer cells.

Key words: pancreatic cancer, miR-590-3p, invasion, metastasis

中图分类号:

R657.5

王亚东, 王俊峰. 上调miR-590-3p对胰腺癌细胞侵袭转移的作用及影响机制[J]. 中华养生保健, 2023, 41(23): 4-8.

WANG Ya-dong, WANG Jun-feng. Effect of Up Regulating miR-590-3p on Invasion and Metastasis of Pancreatic Cancer Cells and Its Mechanism[J]. ZHONGHUA YANGSHENG BAOJIAN, 2023, 41(23): 4-8.

参考文献

[1] GORAL V.Pancreatic Cancer: Pathogenesis and Diagnosis[J]. Asian Pac J Concer Pre,2015,16(14):5619-5624.
[2] KLEIN A P.Pancreatic cancer epidemiology: understanding the role of lifestyle and inherited risk factors[J]. Nat Rev Gastroenterol Hepatol,2021,18(7):493-502.
[3] ZHAO Z, LIU W.Pancreatic Cancer: A Review of Risk Factors, Diagnosis, and Treatment[J]. Technol Cancer Res Treat,2020,19:1533033820962117.
[4] HILL M, TRAN N. miRNA interplay: mechanisms and consequences in cancer [J]. Disease Models & Mechanisms,2021,14(4):dmm047662.
[5] DIENER C, KELLER A, MEESE E.Emerging concepts of miRNA therapeutics: from cells to clinic[J]. Trends in genetics: TIG,2022,38(6):613-626.
[6] ZHENG Z Q, LI Z X, ZHOU G Q, et al.Long Noncoding RNA FAM225A Promotes Nasopharyngeal Carcinoma Tumorigenesis and Metastasis by Acting as ceRNA to Sponge miR-590-3p/miR-1275 and Upregulate ITGB3[J]. Cancer Research, 2019,79(18):4612-4626.
[7] WANG Y, DING N, GUAN G, et al.Rapid Delivery of Hsa-miR-590-3p Using Targeted Exosomes to Treat Acute Myocardial Infarction Through Regulation of the Cell Cycle[J]. Journal of Biomedical Nanotechnology
2018,14(5):968-977.
[8] SUN Z Q, SHI K, ZHOU Q B, et al.MiR-590-3p promotes proliferation and metastasis of colorectal cancer via Hippo pathway[J]. Oncotarget
2017,8(35):58061-58071.
[9] TORPHY R J, FUJIWARA Y, SCHULICK R D.Pancreatic cancer treatment: better, but a long way to go[J]. Surgery Today,2020,50(10):1117-1125.
[10] ZHANG L, SANAGAPALLI S, STOITA A.Challenges in diagnosis of pancreatic cancer[J]. World Journal of Gastroenterology,2018,24(19): 2047-2060.
[11] WU Y, GU Y, SHI B. miR-590-3p Alleviates diabetic peripheral neuropathic pain by targeting RAP1A and suppressing infiltration by the T cells[J]. Acta Biochimica Polonica,2020,67(4):587-593.
[12] ZHOU W, LIU L, XUE Y, et al.Combination of Endothelial-Monocyte-Activating Polypeptide-II with Temozolomide Suppress Malignant Biological Behaviors of Human Glioblastoma Stem Cells via miR-590-3p/MACC1 Inhibiting PI3K/AKT/mTOR Signal Pathway[J]. Fronti Mol Neurosci,2017,10:68.
[13] WAN Z, LI X, LUO X, et al.The miR-590-3p/CFHR3/STAT3 signaling pathway promotes cell proliferation and metastasis in hepatocellular carcinoma[J]. Aging,2022,14(14):5783-5799.
[14] HUANG M, LONG Y, JIN Y, et al.Comprehensive analysis of the lncRNA-miRNA-mRNA regulatory network for bladder cancer[J]. Translational Andrology and Urology,2021,10(3):1286-1301.
[15] WU H J, ZHANG K, MA J J, et al.Mechanism of curcumin against myocardial ischaemia-reperfusion injury based on the P13K/Akt/mTOR signalling pathway[J]. European Review for Medical and Pharmacological Sciences,2021,25(17): 5490-5499.
[16] LI Z, SONG Y, HOU W, et al.Atractylodin induces oxidative stress-mediated apoptosis and autophagy in human breast cancer MCF-7 cells through inhibition of the P13K/Akt/mTOR pathway[J]. Journal of Biochemical and Molecular Toxicology,2022,36(8):e23081.
[17] TANG X Y, LUO Z L, XIONG Y L, et al.The Proliferative Role of Immune Checkpoints in Tumors: Double Regulation[J]. Cancers,2022,14(21):5374.
[18] GASPARRI M L, BESHARAT Z M, FAROOQI A A, et al.MiRNAs and their interplay with PI3K/AKT/mTOR pathway in ovarian cancer cells: a potential role in platinum resistance[J]. J Cancer Res Clini Oncol,2018,144(12):2313-2318.