慢性萎缩性胃炎向胃癌转变的生物信息学分析及中药预测研究

中华养生保健 ›› 2023, Vol. 41 ›› Issue (19): 69-75.

慢性萎缩性胃炎向胃癌转变的生物信息学分析及中药预测研究

孙启芳^1,2, 谭美玲^1,2, 袁承志^1,3, 周赛男⁴, 曾梅艳², 宋厚盼^1,2,*

1.湖南中医药大学中医诊断学湖南省重点实验室,湖南长沙,410208;
2.湖南中医药大学中医学院,湖南长沙,410208;
3.湖南中医药大学医学院,湖南长沙,410208;
4.湖南中医药大学第一附属医院脾胃病科,湖南长沙,410021

出版日期:2023-10-01 发布日期:2023-09-20
通讯作者: ^*宋厚盼,E-mail：hpsong2015@126.com。
作者简介:孙启芳（2003—）,女,汉族,籍贯：湖南省益阳市,本科在读,研究方向：中医药防治脾胃病。
基金资助:
国家自然科学基金面上项目（82374429）; 湖湘青年科技创新人才项目（2021RC3101）; 湖南省自然科学基金面上项目（2023JJ30460）; 湖南中医药大学中医学一流学科开放基金项目（2018ZYX13）; 湖南省大学生研究性学习和创新性实验计划项目（S202210541099）

Bioinformatics Analysis of the Transformation from Chronic Atrophic Gastritis to Gastric Cancer and Prediction of Traditional Chinese Medicine

SUN Qi-fang^1,2, TAN Mei-ling^1,2, YUAN Cheng-zhi^1,3, ZHOU Sai-nan⁴, ZENG Mei-yan², SONG Hou-pan^1,2,*

1. Hunan Provincial Key Laboratory of Diagnostic Research in Chinese Medicine, Hunan University of Chinese Medicine, Changsha Hunan 410208, China;
2. Faculty of Medicine, Hunan University of Chinese Medicine, Changsha Hunan 410208, China;
3. Faculty of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha Hunan 410208, China;
4. Department of Spleen and Gastroenterology, The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha Hunan 410021, China

Online:2023-10-01 Published:2023-09-20

摘要/Abstract

摘要： 目的检索慢性萎缩性胃炎与胃癌的差异表达基因,探究胃癌转变的分子机制,发掘具有潜在治疗作用的中药及中药活性成分。方法在GEO数据库下载GSE116312基因芯片数据集,得到差异表达基因,通过Metascape数据库进行GO和KEGG通路富集,采用String数据库构建差异表达基因蛋白交互作用网络以明确核心基因,分析核心基因的预后及免疫细胞浸润相关性,并应用Coremine数据库及CTD数据库分别获得具有潜在治疗作用的中药与中药活性成分。结果共获得显著性差异表达基因129个,其中上调105个,下调24个。生物过程主要包括消化、激素调节、对细菌的反应、器官或组织特异性免疫反应、对异源刺激的反应等,富集于蛋白质消化吸收、癌症转录失调、胃酸分泌、果糖和甘露糖代谢、脂肪消化吸收等通路。MMP3、CTGF、THBS1、SPP1、IL6、CXCL8、END1、BGN、THBS2、MUC6是慢性萎缩性胃炎向胃癌转化的核心基因,核心基因与免疫细胞浸润存在较高相关性,其中MUC6与THBS1高表达患者的生存率明显低于低表达患者,MMP3与CTGF高表达患者的生存率高于低表达患者。发掘出人参、三七、茵陈、厚朴、茯苓、丹参、白花蛇舌草、枸杞子等潜在治疗中药,筛选出白藜芦醇、槲皮素、紫杉醇、穿心莲内酯等治疗胃癌的潜在靶向中药活性成分。结论本研究发现的慢性萎缩性胃炎与胃癌的核心差异表达基因与通路为后续基础实验和临床研究提供了参考,并为运用中医药阻断炎癌转变提供了思路。

关键词: 慢性萎缩性胃炎, 胃癌, 生物信息学, 差异基因, 中药

Abstract: Objective To search the differentially expressed genes between chronic atrophic gastritis and gastric cancer, explore the molecular mechanism of gastric cancer transformation, and explore the potential therapeutic effects of traditional Chinese medicine and active components of traditional Chinese medicine. Methods The GSE116312 gene chip dataset was downloaded from the GEO database, and the differentially expressed genes were obtained. The GO and KEGG pathways were enriched through the Metascape database. The String database was used to construct the differentially expressed gene-protein interaction network to identify key genes, and to analyze the prognosis and immunity of key genes. The Coremine database and the CTD database were used to obtain Chinese herbal medicines and active ingredients with potential therapeutic effects, respectively. Results A total of 129 significantly differentially expressed genes were obtained, of which 105 were up-regulated and 29 were down-regulated. Biological processes mainly include digestion, hormone regulation, response to bacteria, organ or tissue specific immune response, response to heterogeneous stimuli, etc. They are enriched in protein digestion and absorption, cancer transcriptional disorder, gastric acid secretion, fructose and mannose metabolism, fat digestion and absorption and other pathways. MMP3, CTGF, THBS1, SPP1, IL6, CXCL8, END1, BGN, THBS2, and MUC6 are key genes in the transformation of chronic atrophic gastritis to gastric cancer. There is a high correlation between key genes and immune cell infiltration. The survival rate of patients with high expression of MUC6 and THBS1 is significantly lower than that of patients with low expression, and the survival rate of patients with high expression of MMP3 and CTGF is higher than that of patients with low expression. The potential therapeutic traditional Chinese medicines such as ginseng, panax notoginseng, herba artemisiae, magnolia officinalis, poria cocos, salvia miltiorrhiza, hedyotis diffusa, lycium barbarum were discovered, and the potential targeted active ingredients of traditional Chinese medicines such as resveratrol, quercetin, paclitaxel, andrographolide for treating gastric cancer were screened. Conclusion The core differentially expressed genes and pathways of chronic atrophic gastritis and gastric cancer discovered in this study provide a reference for subsequent basic experiments and clinical research, and provide ideas for the use of traditional Chinese medicine to block the transformation of inflammation and cancer.

Key words: chronic atrophic gastritis, gastric cancer, bioinformatics, differential genes, traditional Chinese medicine

中图分类号:

R573.3

孙启芳, 谭美玲, 袁承志, 周赛男, 曾梅艳, 宋厚盼. 慢性萎缩性胃炎向胃癌转变的生物信息学分析及中药预测研究[J]. 中华养生保健, 2023, 41(19): 69-75.

SUN Qi-fang, TAN Mei-ling, YUAN Cheng-zhi, ZHOU Sai-nan, ZENG Mei-yan, SONG Hou-pan. Bioinformatics Analysis of the Transformation from Chronic Atrophic Gastritis to Gastric Cancer and Prediction of Traditional Chinese Medicine[J]. ZHONGHUA YANGSHENG BAOJIAN, 2023, 41(19): 69-75.

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