参苓白术散对腹泻幼鼠模型肠黏膜通道蛋白的影响及机制研究

摘要/Abstract

摘要： 目的探讨参苓白术散对腹泻幼鼠模型肠黏膜通道蛋白的影响及机制。方法将腹泻幼鼠模型（n=42,采用番泻叶药液灌胃进行建模）随机平分为三组,分别为模型组、低剂量参苓白术散组、高剂量参苓白术散组,三组分别给予参苓白术散0.0 g/kg（即0.9%氯化钠溶液）、1.2 g/kg、4.6 g/kg灌胃治疗,给药体积均为10 mL/kg,连续用药14 d,检测幼鼠肠黏膜通道蛋白-水通道蛋白8（AQP8）、跨膜转运蛋白钠氢交换体3（NHE3）囊性纤维化跨膜传导调节因子（CFTR）表达变化情况,观察幼鼠体质量、尿液乳果糖和甘露醇比值、小肠组织绒毛长度等变化情况。结果低剂量参苓白术散组、高剂量参苓白术散组治疗第7天、第14天的幼鼠体质量明显高于模型组（P<0.05）,腹泻指数与模型组相比明显降低（P<0.05）,高剂量参苓白术散组与低剂量参苓白术散组对比,差异有统计学意义（P<0.05）。低剂量参苓白术散组、高剂量参苓白术散组治疗第7天、第14天的尿液乳果糖和甘露醇比值明显低于模型组（P<0.05）,高剂量参苓白术散组也低于低剂量参苓白术散组（P<0.05）。低剂量参苓白术散组、高剂量参苓白术散组治疗第14天的小肠组织绒毛长度高于模型组（P<0.05）,隐窝深度明显低于模型组（P<0.05）,高剂量参苓白术散组与低剂量参苓白术散组对比,差异有统计学意义（P<0.05）。低剂量参苓白术散组、高剂量参苓白术散组治疗第14天的小肠组织AQP8蛋白、NHE3蛋白相对表达水平高于模型组（P<0.05）,CFTR蛋白相对表达水平低于模型组（P<0.05）,低剂量参苓白术散组与高剂量参苓白术散组对比,差异有统计学意义（P<0.05）。结论参苓白术散在腹泻幼鼠模型中的应用能促进体质量的恢复,降低尿液乳果糖和甘露醇比值与腹泻指数,还可延长小肠组织绒毛长度与降低隐窝深度,同时可促进小肠组织AQP8蛋白、NHE3蛋白的表达,抑制CFTR蛋白的表达。

关键词: 参苓白术散, 腹泻, 幼鼠模型, 乳果糖和甘露醇比值, 跨膜转运蛋白钠氢交换体3, 水通道蛋白8

Abstract: Objective To explore and analysis the effects and mechanism of Shenling Baizhu Powder on intestinal mucosal channel proteins in a young mouse model of diarrhea. Methods Young rats with diarrhea (n=42) were randomly equally divided into three groups - model group, low-dose Shenling Baizhu Powder group, high-dose Shenling Baizhu Powder group. The three groups were given Shenling Baizhu Powder 0.0 g/kg(i.e. 0.9% sodium chloride solution), 1.2 g/kg, 4.6 g/kg by gavage, with the dose volume of 10 mL/kg, for 14 consecutive days. The expression of intestinal mucosal channel AQP8, CFTR in transmembrane transport protein NHE3 were detected. Results The body weight of young mice in the low-dose Shenling Baizhu Powder group and high-dose Shenling Baizhu Powder group on the 7th and 14th day of treatment were significantly higher than that in the model group (P<0.05), and the diarrhea index were significantly reduced compared to the model group (P<0.05). There were also a statistical significant difference between the high-dose Shenling Baizhu Powder group and the low-dose Shenling Baizhu Powder group (P<0.05). The ratio of urine Lactulose and Mannitol in the low-dose and high-dose Shenling Baizhu Powder groups were significantly lower than that in the model group on the 7th and 14th days of treatment (P<0.05), and the high-dose Shenling Baizhu Powder group were also lower than that in the low-dose Shenling Baizhu Powder group (P<0.05). On the 14th day of treatment, the villus length of small intestine tissue in the low-dose and high-dose Shenling Baizhu Powder groups were higher than that in the model group (P<0.05), and the crypt depth were significantly lower than that in the model group (P<0.05). There were also statistical significant difference compared between the high-dose and low-dose Shenling Baizhu Powder groups(P<0.05). On the 14th day of treatment, the relative expression levels of AQP8 protein and NHE3 protein in the small intestine tissue of the low-dose and high-dose Shenling Baizhu Powder groups were higher than those of the model group (P<0.05), while the relative expression levels of CFTR protein were lower than those of the model group (P<0.05). There were also significant difference compared between the low-dose and high-dose Shenling Baizhu Powder groups (P<0.05). Conclusion Shenling Baizhu Powder can promote the recovery of body weight, reduce the ratio of Lactulose and Mannitol in urine and diarrhea index, prolong the villus length and reduce the recess depth of small intestine, promote the expression of AQP8 protein and NHE3 protein in small intestine, and inhibit the expression of CFTR protein.

Key words: Shenling Baizhu Powder, diarrhea, mouse model, ratio of lactulose to mannitol, transmembrane transport protein sodium hydrogen exchanger 3, Aquaporin 8

中图分类号:

R365

马玮玮, 王彦彬, 王晓颖, 徐国娟, 梁翠才, 戚敏敏, 尉家森. 参苓白术散对腹泻幼鼠模型肠黏膜通道蛋白的影响及机制研究[J]. 中华养生保健, 2024, 42(13): 14-18.

MA Wei-wei, WANG Yan-bin, WANG Xiao-ying, XU Guo-juan, LIANG Cui-cai, QI Min-min, WEI Jia-sen. Study on the Effect and Mechanism of Shenling Baizhu Powder on Intestinal Mucosal Channel Proteins in a Juvenile Rat Model of Diarrhea[J]. ZHONGHUA YANGSHENG BAOJIAN, 2024, 42(13): 14-18.

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