Fas/FasL传导通路研究电针对慢性萎缩性胃炎小鼠的作用机制

摘要/Abstract

摘要： 目的探讨电针合募配穴对慢性萎缩性胃炎小鼠Fas/FasL传导通路的影响,揭示电针治疗慢性萎缩性胃炎的作用机制。方法制备慢性萎缩性胃炎小鼠模型。将已成模的慢性萎缩性胃炎小鼠分为模型组10只、普通针刺组、电针组各20只,并另设10只为对照组。对照组、模型组不进行操作;普通针刺组予合募配穴（中脘-足三里）普通针刺治疗;电针组予合募配穴（中脘-足三里）电针治疗,采用免疫组化法检测各组小鼠胃组织中Fas和FasL的表达水平。结果治疗前普通针刺组、电针组、模型组的体质量都显著低于对照组（P<0.05）,治疗14 d后普通针刺组、电针组的体质量高于模型组（P<0.05）,电针组治疗14 d后的体质量与对照组比较,差异无统计学意义（P>0.05）。治疗前普通针刺组、电针组、模型组的血清IL-6、IL-1β含量都高于对照组（P<0.05）,治疗14 d后普通针刺组、电针组的血清IL-6、IL-1β含量低于模型组（P<0.05）,电针组治疗14 d后的血清IL-6、IL-1β含量与对照组比较,差异无统计学意义（P>0.05）。治疗14 d后普通针刺组、电针组、对照组的胃黏膜组织病理形态评分与黏膜组织Fas蛋白、FasL蛋白相对表达水平都低于模型组（P<0.05）,电针组与对照组比较,差异无统计学意义（P>0.05）。结论电针在慢性萎缩性胃炎小鼠的应用能介导Fas/FasL传导通路的表达,抑制血清IL-6、IL-1β的表达,促进恢复小鼠的体质量,能改善小鼠的胃黏膜病理状况。

关键词: 慢性萎缩性胃炎, 电针, 合募配穴, 小鼠, Fas/FasL传导通路

Abstract: Objective To explore the effects of electroacupuncture combined with acupoints Mu on Fas/FasL conduction pathway in mice with chronic Atrophic gastritis, and reveal the mechanism of electroacupuncture in treating chronic Atrophic gastritis. Methods The mouse model of chronic Atrophic gastritis were established. The modeled chronic Atrophic gastritis mice were divided into the model group of 10 cases, the common acupuncture group of 20 cases, and the electroacupuncture group of 20 cases, and another 10 cases of the control group. The control group and model group were not operated; The ordinary acupuncture group were treated with combined acupuncture at acupoints (Zhongwan Zusanli); The electroacupuncture group were treated with acupoint combination (Zhongwan Zusanli) electroacupuncture. Immunohistochemical methods were used to detect the levels of Fas and FasL in the gastric tissues of each groups of mice. Results The body weight of the ordinary acupuncture group, electroacupuncture group, and model group before treatment were significantly lower than that of the control group (P<0.05). After 14 days of treatment, the body weight of the ordinary acupuncture group and electroacupuncture group were higher than that of the model group (P<0.05). There were no significant difference in body weight between the electroacupuncture group and the control group after 14 days of treatment (P>0.05). Serum IL-6 and IL-1β in the ordinary acupuncture group, electroacupuncture group, and model group before treatment were higher than those in the control group (P<0.05). After 14 days of treatment, the serum IL-6 and IL-1β levels in the ordinary acupuncture group and the electroacupuncture group were significantly higher than those in the control group, and were lower than that of the model group (P<0.05), and the serum IL-6 and IL-1β levels in the ordinary acupuncture group after 14 days of treatment were lower than those in the model group (P<0.05), There were no significant difference in the electroacupuncture group of treatment compared to the control group (P>0.05). After 14 days of treatment, the histopathological score of Gastric mucosa and the relative expression level of Fas protein and FasL protein of mucosa in the ordinary acupuncture group, the electroacupuncture group and the control group were lower than those in the model group (P<0.05), and there were no significant difference compared between the electroacupuncture group and the control group (P>0.05). Conclusion The application of electroacupuncture in chronic Atrophic gastritis mice can mediate the expression of Fas/FasL conduction pathway, and can inhibit the serum IL-6, IL-1 β The expression of can also promote the recovery of mouse weight and improve the pathological condition of the gastric mucosa in mice.

Key words: chronic atrophic gastritis, electroacupuncture, collaborating and matching acupoints, mice, Fas/FasL conduction pathway

中图分类号:

R246

王树国, 薛晓捷, 张春龙. Fas/FasL传导通路研究电针对慢性萎缩性胃炎小鼠的作用机制[J]. 中华养生保健, 2024, 42(2): 17-21.

WANG Shu-guo, XUE Xiao-jie, ZHANG Chun-long. Fas/FasL Conduction Pathway Studies the Mechanism of Electroacupuncture on Chronic Atrophic Gastritis in Mice[J]. ZHONGHUA YANGSHENG BAOJIAN, 2024, 42(2): 17-21.

参考文献

[1] 刘长发,于国强,李丹丹,等.电针结合丹芪祛瘀止痛颗粒治疗萎缩性胃炎[J].吉林中医药,2021,41(7):960-963.
[2] 荣华,付永民,杜旭.丹石健脾汤联合针灸治疗慢性萎缩性胃炎胃癌前病变43例[J].环球中医药,2023,16(4):765-768.
[3] 刘燕观,卢利军.母子补泻针法结合艾灸治疗浅表性胃炎临床研究[J].陕西中医,2022,43(10):1467-1469,1480.
[4] 毕学琦,夏先根,熊琴,等.腹针联合中药治疗幽门螺杆菌阳性的萎缩性胃炎疗效观察[J].上海针灸杂志,2023,42(4):358-362.
[5] 丁亮,肖燕.半夏泻心汤配合针刺治疗寒热错杂型慢性萎缩性胃炎的效果[J].中国医药导报,2021,18(35):167-170.
[6] 李琪,吴梦蝶,吴焕淦,等.针灸治疗慢性萎缩性胃炎临床随机对照试验研究综述[J].世界科学技术-中医药现代化,2019,21(8):1563-1572.
[7] 王晓霞,杨改琴,左甲,等.长针深刺背俞穴治疗慢性萎缩性胃炎的临床研究[J].河北中医,2018,40(3):421-425.
[8] 范丽红,方园,黄河,等.针刺与艾灸治疗慢性萎缩性胃炎的多中心前瞻性临床研究[J].湖南中医药大学学报,2020,40(9):1060-1064.
[9] 陈婕,唐纯志,关凤仪.针刺联合穴位埋线治疗脾胃虚弱型慢性萎缩性胃炎临床观察[J].西部中医药,2019,32(4):115-117.
[10] 车红霞,李嘉,犹春丽,等.针灸治疗慢性非萎缩性胃炎虚证的选穴规律探析[J].中医药通报,2022,21(2):46-49.
[11] 卢泳,王家涛,陈日新.针药结合治疗慢性萎缩性胃炎肠化生的疗效观察[J].中国针灸,2005,25(7):457-459.
[12] 丁霞,李园,沈洪.从精准医学的视角看中医药防治慢性胃炎”炎癌转化”[J].北京中医药大学学报,2023,46(3):315-320.
[13] 沈春霞,王彦刚.基于核心病机观浅析王彦刚论治慢性糜烂性胃炎经验拾萃[J].世界中西医结合杂志,2023,18(4):676-680,713.
[14] 张娟,邱玉萍,罗晶,等.鸡胵养胃散治疗慢性萎缩性胃炎的效果与作用机制探讨[J].中国医药,2023,18(5):736-740.
[15] 郭红伟,程艳丽.基于iTRAQ技术的阿司匹林相关胃黏膜病变差异蛋白质组学研究[J].胃肠病学和肝病学杂志,2023,32(4):361-366.
[16] 邢建菲,苏倩奕,吴海新,等.中医外治法在慢性萎缩性胃炎中的应用研究进展[J].世界中医药,2023,18(1):137-140.
[17] 李昊天,谢晶日,孙志文.谢晶日教授运用调肝通胃法论治慢性萎缩性胃炎经验探析[J].浙江中医药大学学报,2023,47(2):161-166.
[18] 秦宇宁,赵天易,刘凤斌,等.中文版胃肠道症状评定量表对胃肠道疾病患者测量学特性研究[J].中国全科医学,2023,26(18):2277-2285.
[19] 江姗姗,汪红娟,唐红,等.”或针吐,中脘气海膻中补”理论探析[J].广州中医药大学学报,2022,39(9):2180-2183.
[20] 李楠,王捷虹,穆恒.王捷虹教授基于”心脾同治法”论治慢性萎缩性胃炎伴焦虑障碍经验撷英[J].现代中西医结合杂志,2022,31(21):2980-2984.