食管癌组织中FHIT蛋白及mRNA的临床意义分析

摘要/Abstract

摘要： 目的分析食管癌组织中脆性组氨酸三联体基因（fragilehistidinetriad,FHIT）蛋白及mRNA的表达及临床意义。方法回顾性选取2019年3月~2021年10月赤峰市医院的食管癌患者60例样本为病例组、内镜室的正常食管组织患者60例样本作为对照组。统计分析两组组织中FHIT蛋白及mRNA表达,分析病例组癌组织中FHIT mRNA与临床病理参数的相关性。结果与对照组正常组织相比,病例组癌组织、癌旁组织中FHIT蛋白缺失表达率、弱表达率均较高,正常表达率均较低,差异有统计学意义（P<0.05）,而与癌旁组织相比,癌组织中FHIT蛋白缺失表达率较高,弱表达率、正常表达率均较低,差异有统计学意义（P<0.05）。与对照组正常组织相比,病例组癌组织、癌旁组织中FHIT mRNA缺失率均较高,差异有统计学意义（P<0.05）,与癌旁组织相比,癌组织中FHIT mRNA缺失率较高,差异有统计学意义（P<0.05）。与Ⅰ期患者相比,病例组Ⅱ+Ⅲ+Ⅳ期患者的FHIT蛋白缺失率及mRNA缺失率较高,差异有统计学意义（P<0.05）;与高分化患者相比,低分化、中分化患者的FHIT蛋白缺失率及mRNA缺失率均较高,差异有统计学意义（P<0.05）;与无淋巴结转移患者相比,有淋巴结转移患者的FHIT蛋白缺失率及mRNA缺失率较高,差异有统计学意义（P<0.05）。结论 FHIT蛋白及mRNA在食管癌组织中的缺失表达率提升,能够为临床诊断与治疗及预后判断提供有效依据。

关键词: 食管癌, FHIT, 蛋白, mRNA, 临床病理参数

Abstract: Objective To analyze the expression and clinical significance of FHIT protein and mRNA in esophageal carcinoma. Methods A total of 60 samples from patients with esophageal cancer in Chifeng Hospital from March 2019 to October 2021 were retrospectively selected as the case group and 60 samples from patients with normal esophageal tissue in the endoscopy room were selected as the control group. The expression of FHIT protein and mRNA in the two groups were statistically analyzed, and the correlation between FHIT mRNA in the case group and clinicopathological parameters was analyzed. Results Compared with the normal tissues of the control group, the deletion and weak expression rates of FHIT protein in the cancer tissues and adjacent tissues of the case group were higher, and the normal expression rates were lower, the diffierence was statistically signicant (P<0.05). Compared with adjacent tissues, the expression rate of FHIT protein deletion in cancer tissues was higher, and the weak and normal expression rates were lower, the diffierence was statistically signicant (P<0.05). Compared with the normal tissue of the control group, the deletion rate of FHIT mRNA in the cancer tissue and paracancerous tissue of the case group was higher, the diffierence was statistically signicant (P<0.05), and compared with the paracancerous tissue, the deletion rate of FHIT mRNA in the cancer tissue was higher, the diffierence was statistically signicant (P<0.05). Compared with patients with stage I, the FHIT protein deletion rate and mRNA deletion rate of patients with stage Ⅱ+Ⅲ+Ⅳ in the case group were higher, the diffierence was statistically signicant (P<0.05). Compared with well-differentiated patients, the expression rate of FHIT protein deletion and mRNA deletion rate were higher in patients with low and medium differentiation, the diffierence was statistically signicant (P<0.05). Compared with patients without lymph node metastasis, the FHIT protein deletion rate and mRNA deletion rate of patients with lymph node metastasis were higher, the diffierence was statistically signicant (P<0.05). Conclusion The loss expression rate of FHIT protein and mRNA in esophageal carcinoma tissues is increased, which can provide effective basis for clinical diagnosis, treatment and prognosis.

Key words: esophageal carcinoma, FHIT, protein, mRNA, clinicopathological parameters

中图分类号:

R735.1

陈鹏. 食管癌组织中FHIT蛋白及mRNA的临床意义分析[J]. 中华养生保健, 2023, 41(2): 9-12.

CHEN Peng. Clinical Significance of FHIT Protein and mRNA in Esophageal Carcinoma Dodson TB[J]. ZHONGHUA YANGSHENG BAOJIAN, 2023, 41(2): 9-12.

参考文献

[1] 刘玲,李惠武,王延蛟,等.新疆哈萨克族食管癌FHIT基因和MGMT基因启动子区CpG岛甲基化程度及临床意义[J].武汉大学学报(医学版),2019,40(3):372-378.
[2] 李卉,刘玲,王洪江,等.高分辨熔解曲线法检测哈萨克族食管癌患者癌组织中FHIT、CDKN2A启动子区甲基化水平及临床意义[J].科技导报,2011,29(31):59-63.
[3] 李磊,邓彦超,赵燕,等.新疆哈萨克族和汉族食管癌患者FHIT基因甲基化及其与预后的关系[J].癌变·畸变·突变,2012,24(2):132-135.
[4] 张德庆,陈东育,宋兆峰.FHIT蛋白及mRNA在食管癌组织中的表达及意义[J].中国实验诊断学,2011,15(1):83-85.
[5] 中国中西医结合学会.中西医结合食管癌治疗方案专家共识(2021年版)[J].中日友好医院学报,2021,35(1):3-7.
[6] 朱佳龙,李锋,尹来波,等.食管癌遗传易感基因的研究进展[J].现代生物医学进展,2012,12(7):1398-1400.
[7] 陈艳,邓彦超,黄思语.甲基硝基亚硝基胍对哈萨克族正常食管上皮细胞LRRFIP1、ALDH1L1基因甲基化及其表达的影响[J].新疆医科大学学报,2015,14(5):552-557.
[8] 安琪,张丽英,叶菁,等.FHIT基因及其与食管癌关系的研究进展[J].现代肿瘤医学,2010,18(4):825-827.
[9] 张德庆,陈东育,宋兆峰.FHIT、Survivin基因在食管癌组织中的表达及相关性研究[J].中国实验诊断学,2009,13(11):1549-1551.
[10] 苏乃伟,覃家锦,冯旭.广西壮族食管癌患者FHIT基因表达及与Bcl-2表达的相关性研究[J].广西医学,2009,31(1):1-3.
[11] 邓伟明,李道航,孟凡亮.食管癌组织miR-122-5p、CREB1 mRNA表达变化及其临床意义[J].山东医药,2022,62(1):10-14.
[12] 陈昱名,徐登飞,冯浩,等.肌球蛋白重链9在胃癌组织中的表达及对胃癌细胞增殖能力的影响[J].中华实用诊断与治疗杂志,2022, 36(3):231-236.
[13] 何章彪,王翠侠,韩文杰,等.MTSS1及Gli1在食管鳞癌组织中表达及其相关性[J].中国老年学杂志,2022,42(11):2780-2783.
[14] 山周琦玥,周益臣,朱欣欣,等.选择性自噬接头蛋白、微管相关蛋白轻链3和信号转导与转录激活因子3在食管鳞癌中的表达及临床意义[J].中华实验外科杂志,2022,39(4):729-732.
[15] 周超锋,周世繁,田青,等.lncRNA-NORAD表达对食管癌Eca-109细胞生物学行为的影响及其机制[J].吉林大学学报(医学版),2022, 48(1):33-43.
[16] 庹晓霞. 食管癌组织中TROP2的表达水平及临床意义[J].国际检验医学杂志,2020,41(14):1720-1724.
[17] 冶俊玲,苟笑丹,韩静绮,等.BDNF和TrkB在食管癌组织中表达及BDNF对食管癌细胞生物学行为的影响[J].中国老年学杂志,2020, 40(9):1828-1832.
[18] 彭蓉,娜吧错,益西卓呷,等.不同病理类型食管癌GRP78、GRP94表达及与预后的关系分析[J].解放军医药杂志,2020,32(7):22-26.
[19] 方兰,卢一艳,刘金楠.CDCA3在食管癌中的表达及其对食管癌细胞增殖与凋亡的影响[J].国际检验医学杂志,2020,41(5):583-587,591.
[20] 田丽贞,韩润焜,田丽如.食管癌血清标志物的生物信息学筛选及其临床诊断价值验证[J].转化医学杂志,2020,9(6):351-355.