昆布多糖对动脉粥样硬化性ApoE-/-小鼠的辅助降血脂作用机制

中华养生保健 ›› 2025, Vol. 43 ›› Issue (3): 11-15.

昆布多糖对动脉粥样硬化性ApoE-/-小鼠的辅助降血脂作用机制

徐新颖^1,4, 刘姿杉^2,4, 倪钦帅^2,4, 于竹芹^3,4,*, 周缜⁴

1.济南市济阳区中医医院ICU,山东济南, 251400;
2.青岛大学附属海慈医院神经科,山东青岛, 266023;
3.青岛市第六人民医院内科,山东青岛, 266023;
4.青岛大学医学部中西医结合中心,山东青岛, 266021

出版日期:2025-02-01 发布日期:2025-02-10
通讯作者: ^*于竹芹,E-mail：yuzhuq2008@163.com。
作者简介:徐新颖（1983—）,女,汉族,籍贯：山东省济南市,硕士研究生,主治医师,研究方向：中西医结合临床。
基金资助:
山东省中医药科技项目（M-2022004）; 青岛市中医药科研计划项目（2020-ZYZ003）

Auxiliary Hypolipidemic Effect and Mechanism of Laminaria Polysaccharide in ApoE-/- Mice of Atherosclerosis

XU Xin-ying^1,4, LIU Zi-shan^2,4, NI Qin-shuai^2,4, YU Zhu-qin^3,4,*, ZHOU Zhen⁴

1. Department of ICU, Jiyang District TCM Hospital, Jinan Shandong, 251400, China;
2. Department of Neurology, Hiser Hospital Affiliated to Qingdao University, Qingdao Shandong, 266023, China;
3. Department of Medicine, The Sixth Peoples' Hospital of Qingdao, Qingdao Shandong, 266023, China;
4. Institute of Integrative Medicine, Qingdao University Medical College, Qingdao Shandong, 266021, China

Online:2025-02-01 Published:2025-02-10

摘要/Abstract

摘要： 目的研究昆布多糖在动脉粥样硬化性ApoE-/-小鼠的辅助降血脂及其可能的机制。方法应用高脂饲料喂养法建立动脉粥样硬化性ApoE-/-小鼠模型,随机分为模型组、阳性药组、昆布多糖低、中、高剂量组,普通饲料喂养C57BL/6J小鼠为对照组。治疗组给予昆布多糖（1 g/kg、2 g/kg、4 g/kg）灌胃,隔日1次;阳性药组给予腹腔注射波立达（10 mg/kg）,1次/周,疗程4周;对照组和模型组小鼠同步给予等容积的0.9%氯化钠溶液,疗程4周。应用生化法检测小鼠血清甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇C（LDL-C）和高密度脂蛋白胆固醇C（HDL-C）水平,酶联免疫吸附法检测小鼠血清瘦素（LEP）和脂联素（APN）水平,苏木素尹红（HE）染色观察小鼠肝组织的脂质沉积。结果治疗结束后与模型组比较,昆布多糖组和阳性药组小鼠体质量均明显下降（P<0.05）,血清TG、TC、LDL-C含量均明显降低（P<0.05）、HDL-C水平均明显升高（P<0.05）,血清LEP和APN水平均显著升高（P<0.05）,肝组织脂质沉积均显著改善。结论昆布多糖能改善动脉粥样硬化小鼠的脂质代谢,其作用机制可能与提高血清LEP和APN水平有关。

关键词: 昆布多糖, 动脉粥样硬化, 血脂, 瘦素, 脂联素, ApoE-/-小鼠

Abstract: Objective To investigate the helping hypolipidemic effect and possible mechanism of Laminaria polysaccharide in ApoE-/-mice of atherosclerosis. Methods Atherosclerotic ApoE-/-mice models were established by feeding with high-fat diet for 12 weeks and were randomly divided into model group, low-, medium- and high-dose groups and positive drug group, C57BL/6J mice fed with ordinary feed as the control group. After modeling, Laminaria polysaccharide (1 mg/kg, 2 mg/kg, 4 mg/kg) was administrated intragastrically once every other day in the treatment groups, and praluent injection (10 mg/kg) was intraperitoneally injected once a week in the positive drug group for 4 weeks. The mice in the control and model groups were simultaneously given equal volume saline. The serum levels of triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol C (LDL-C) and high-density lipoprotein cholesterol C (HDL-C) were detected by biochemical methods. Enzyme linked immunosorbent assay (ELISA) was used to determine the serum levels of leptin (LEP) and adiponectin (APN), and HE staining to observe the fat deposition in hepatic tissue. Results After the end of the administration cycle, the body weights of mice in the Laminaria polysaccharide and positive drug groups were significantly decreased than those in the model group (P<0.05). The serum levels of TG, TC and LDL-C were significantly decreased (P<0.05) while the HDL-C was significantly increased (P<0.05) than those in the model group. The serum levels of LEP and APN were significantly higher than those in the model group (P<0.05), and the fat deposition in hepatic tissue was significantly improved than model group. Conclusion Laminaria polysaccharide could improve lipid metabolism, which might related to evaluating the serum levels of LEP and APN.

Key words: Laminaria polysaccharide, atherosclerosis, lipids, leptin, adiponectin, ApoE-/-mice

中图分类号:

R285.5

徐新颖, 刘姿杉, 倪钦帅, 于竹芹, 周缜. 昆布多糖对动脉粥样硬化性ApoE-/-小鼠的辅助降血脂作用机制[J]. 中华养生保健, 2025, 43(3): 11-15.

XU Xin-ying, LIU Zi-shan, NI Qin-shuai, YU Zhu-qin, ZHOU Zhen. Auxiliary Hypolipidemic Effect and Mechanism of Laminaria Polysaccharide in ApoE-/- Mice of Atherosclerosis[J]. ZHONGHUA YANGSHENG BAOJIAN, 2025, 43(3): 11-15.

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