三子养亲汤通过调控JAK/STAT信号通路减轻哮喘小鼠肺损伤

摘要/Abstract

摘要： 目的研究三子养亲汤通过调控JAK/STAT信号通路减轻哮喘小鼠肺损伤。方法将50只小鼠随机分为5组：正常组、模型组、阳性组、三子养亲汤低剂量组和高剂量组,使用卵清蛋白复制哮喘小鼠模型。HE染色检测小鼠肺组织病理变化,检测肺功能,使用ELISA检测肺泡灌洗液中IL-6和TNF-α水平,使用Western Blotting 检测肺组织中JAK、p-JAK、STAT3、p-STAT3蛋白表达水平。结果正常组小鼠肺组织结构整齐,肺泡结构清晰,肺泡壁薄,肺泡内无炎症细胞浸润,肺泡上皮间质少见胶原沉积;模型组小鼠肺组结构紊乱,肺泡壁增厚,肺泡内有严重的炎症细胞浸润,肺泡间质有很多胶原沉积;阳性组、三子养亲汤低剂量和高剂量组肺组织的上述病理改善较模型组有改善,且三子养清汤高剂量组肺组织的病理改善最为明显。与正常组比较,模型组小鼠的FEV₁和PEF均显著降低（P<0.05）;与模型组比较,阳性组、三子养亲汤低剂量组和三子养亲汤高剂量组的FEV₁和PEF水平均显著升高（P<0.05）。与正常组比较,模型组小鼠的IL-6、TNF-α、p-JAK、p-STAT3水平均显著升高（P<0.05）;与模型组比较,阳性组、三子养亲汤低剂量组和三子养亲汤高剂量组的IL-6、TNF-α、p-JAK、p-STAT3水平均显著降低（P<0.05）。结论三子养亲汤可以有效的改善哮喘小鼠模型的相关病理变化,其作用机制可能为通过调控JAK/STAT信号通路,而改善肺部的炎症反应。

关键词: 三子养亲汤, 哮喘, 炎症反应, JAK/STAT信号通路

Abstract: Objective To study the effects of Sanzi Yangqin Decoction attenuating lung injury in asthmatic mice by regulating JAK/STAT signaling pathway. Methods Fifty mice were randomly divided into normal group, model group, positive group, low-dose Sanzi Yangqin Decoction group and Sanzi Yangqin Decoction high-dose group. Ovalbumin was used to replicate the asthma mouse model. HE staining was used to detect mouse lung tissue, and to detect lung function.IL-6 and TNF-α levels in BALF were measured by ELISA,The protein expression levels of JAK, p-JAK, STAT3 and p-STAT3 were detected by Western Blotting. Results The normal group of mice has neat organization structure, clear alveolar structure, thin alveolar walls, no inflammatory cell infiltration in the alveoli, and rare collagen deposition in the alveolar epithelium and interstitium. Inflammatory cell infiltration, there is a lot of collagen deposition in the alveolar interstitium. The pathological improvement of the lung tissue in the positive group and the low-dose and high-dose groups of Sanzi Yangqin Decoction was improved compared with the model group, and the pathological improvement of the lung tissue in the high-dose Sanzi Yangqing Decoction group was the most obvious. Compared with the normal group, the FEV1 and PEF of the mice in the model group were significantly decreased (P<0.05); compared with the model group, the FEV1 and PEF of the positive group, the low-dose Sanziyangqin Decoction group and the high-dose Sanziyangqin Decoction group were significantly lower (P<0.05). were significantly increased (P<0.05). Compared with the normal group, the levels of IL-6, TNF-α, p-JAK, and p-STAT3 in the model group were significantly increased (P<0.05). The levels of IL-6, TNF-α, p-JAK and p-STAT3 in the dose group and the high-dose Sanziyangqin decoction group were significantly decreased (P<0.05). Conclusions Sanzi Yangqin Decoction can effectively improve the related pathological changes in asthmatic mouse models, and its mechanism of action may be to improve the inflammatory response of the lungs by regulating the JAK/STAT signaling pathway.

Key words: Sanzi Yangqin Decoction, asthma, inflammatory response, JAK/STAT signaling pathway

中图分类号:

R285.5

徐升. 三子养亲汤通过调控JAK/STAT信号通路减轻哮喘小鼠肺损伤[J]. 中华养生保健, 2023, 41(7): 24-27.

XU Sheng. Sanzi Yangqin Decoction Attenuates Lung Injury in Asthmatic Mice by Regulating JAK/STAT Signaling Pathway[J]. ZHONGHUA YANGSHENG BAOJIAN, 2023, 41(7): 24-27.

参考文献

[1] ZHANG X, ZHONG W, MENG Q, et al.Ambient PM2.5 exposure exacerbates severity of allergic asthma in previously sensitized mice[J]. J Asthma,2015,52(8):785-794.
[2] 陈凌,茅松,朱若尘,等.NLRP3抑制剂MCC950对中性粒细胞性哮喘气道炎症的影响及其作用机制[J].中国生物制品学杂志,2022,35(7):829-835.
[3] 庞亚蓉,席建宏,王志旺,等.磷脂酰肌醇3激酶/蛋白激酶B(PI3K/Akt)信号通路参与哮喘气道炎症反应的研究现状[J].中国临床药理学杂志,2021,37(14):1897-1901.
[4] 唐祖鑫,左雪虹,付娟,等.柴黄清胰活血颗粒对重症急性胰腺炎模型大鼠JAK2/STAT3信号通路的影响[J].中药新药与临床药理,2022,33(8):1055-1062.
[5] 王英,陈秋.基于网络药理学探究三子养亲汤治疗慢性阻塞性肺疾病的作用机制[J].中成药,2022,44(7):2386-2390.
[6] 廖小刚,朱爱勇,王欣国,等.中国成人哮喘患病率的Meta分析[J].中国循证医学杂志,2020,20(10):1164-1172.
[7] YAN C, LUO Q, MA C, et al.A preliminary strategy for screening the combination of active components based on Bu-Shen-Yi-Qi Decoction for bronchial asthma[J]. Traditional Medicine and Modern Medicine,2019, 2(3):135-138.
[8] WANG M H, CHEN C, YEH M L, et al. Using Traditional Chinese Medicine to Relieve Asthma Symptoms: A Systematic Review and Meta-Analysis[J]. The American journal of Chinese medicine,2019,47(8):1659-1674.
[9] WANG Y, CHEN Y J, XIANG C, et al.Discovery of potential asthma targets based on the clinical efficacy of Traditional Chinese Medicine formulas[J]. Journal of Ethnopharmacology,2020,252:112635.
[10] SONG W, ZHENG S, LI M, et al. Linking endotypes to omics profiles in difficult-to-control asthma using the diagnostic Chinese medicine syndrome differentiation algorithm[J]. Journal of Asthma,2020,57(5):532-542.
[11] 谭永强,刘海沛,施燕蓉.运动康复对哮喘患儿嗜酸性粒细胞炎症表达的影响[J].临床儿科杂志,2022,40(8):586-590.
[12] 瞿廷念,黄波,雷丽,等.西替利嗪抑制哮喘小鼠肺组织JAK2-STAT3通路激活及肥大细胞活化[J].细胞与分子免疫学杂志,2022,38(3):193-198.
[13] 赵欢欢,王明航,屠新敏,等.支气管哮喘炎症表型与中医证候的相关性[J].中国老年学杂志,2021,41(1):215-218.
[14] 虢强,朱美意,张杰,等.红景天苷调节CLP诱导的脓毒症小鼠肺纤维化及JAK2/STAT3的活化[J].中国免疫学杂志,2022,38(10):1196-1200.
[15] 李海峰,林一春,陈岸东,等.银胶菊内酯调控JAK/STAT信号通路对LPS诱导的肺泡上皮细胞损伤的影响[J].中国老年学杂志,2022,42(8):1975-1978.