反向离子导入对兔眼前房积血治疗的实验研究

中华养生保健 ›› 2023, Vol. 41 ›› Issue (10): 1-4.

• 论著 • 下一篇

反向离子导入对兔眼前房积血治疗的实验研究

孙守彬¹, 杨洁²

1.哈尔滨医科大学附属第四医院眼科,黑龙江哈尔滨,150001;
2.黑龙江省第二医院耳鼻喉科,黑龙江哈尔滨,150000

出版日期:2023-05-16 发布日期:2023-05-16
作者简介:孙守彬（1981—）,男,汉族,籍贯：山东省德州市,博士研究生,副教授,研究方向：铁死亡、近视防控与治疗。
基金资助:
黑龙江省卫生计生委科研课题（2018-004）; 哈尔滨医科大学附属第四医院优秀青年项目（HYDSYYXQN202021）

Evaluation of Treatment by Reverse Iontophoresis in a Rabbit Hyphema Model Introduction

SUN Shou-bin¹, YANG Jie²

1. Department of Ophthalmology, The Fourth Affiliated Hospital of Harbin Medical University, Harbin Heilongjiang, 150001, China;
2. Department of Otolaryngology, The Second Hospital of Heilongjiang Province, Harbin Heilongjiang, 150000, China

Online:2023-05-16 Published:2023-05-16

摘要/Abstract

摘要： 目的利用反向离子导入经角膜提取兔眼前房内铁离子以治疗前房积血。方法 24只白兔随机分为3组,每组8只。其中,Ⅰ组8只白兔不造模为空白对照组。16只白兔右眼制造前房积血模型,造模后再随机分成Ⅱ、Ⅲ两组,每组8只眼。Ⅱ组为模型对照组,给予0 mA电流强度反向离子导入治疗,观察前房积血的自然变化;Ⅲ组为治疗组,经角膜反向离子电渗技术治疗,每日给予0.4 mA电流强度经角膜反向离子导入技术治疗,15 min/次,持续治疗7 d。利用裂隙灯显微镜观察前房积血的吸收速度,利用电感耦合等离子体发射光谱仪测量各组房水中铁离子的浓度,利用组织学检查观察角膜的病理改变。结果房水中铁离子浓度模型对照组明显高于空白对照组和治疗组,差异有统计学意义（P<0.05）。裂隙灯显微镜观察治疗组前房积血的吸收速度明显优于对照组,组织学检查治疗组角膜炎细胞浸润及铁颗粒沉积明显优于模型组。结论反向离子导入可以使兔眼前房内的铁离子通过角膜排除体外,从而对前房积血治疗具有重要意义。

关键词: 反向离子导入, 前房积血, 铁

Abstract: Objective The purpose of this study was to treat hyphema by using reverse ion introductio to extract iron from anterior chamber of the rabbit cornea. Methods 24 rabbits were randomly divided into three groups, with 8 rabbits in each group. The 8 rabbits in group I were not modeled as the blank control group; The anterior chamber blood model was fabricated in the right eyes of 16 rabbits, and after modeling, they were randomly divided into two groups Ⅱ and Ⅲ with 8 eyes in each group. The group Ⅱ was called model control group, which was given 0 ma current intensity reverse iontophoresis treatment to observe the natural changes of anterior chamber blood volume; The group Ⅲ was treated by transcorneal reverse iontophoresis technique at a current intensity of 0.4 Ma once daily for 15 min for seven days. The absorption velocity of the anterior chamber blood was observed by slit lamp microscopy, the concentration of iron ions in the aqueous humor of each group was measured by inductively coupled plasma optical emission spectrometer, and the pathological changes of the cornea were observed by histological examination. Results The iron concentration in aqueous humor was greatly increased in the modeling group compared with control group, but it was greatly reduced by reverse iontophoresis treatment. Histological examination indicated that inflammatory cell infiltration and iron deposition in the RI treatment group were significantly less than the modeling group. Conclusions Reverse iontophoresis in cornea is a non-invasive and promising approach to extract iron from the anterior chamber to treat hyphema.

Key words: reverse iontophoresis, hyphema, iron

中图分类号:

R779.1

孙守彬, 杨洁. 反向离子导入对兔眼前房积血治疗的实验研究[J]. 中华养生保健, 2023, 41(10): 1-4.

SUN Shou-bin, YANG Jie. Evaluation of Treatment by Reverse Iontophoresis in a Rabbit Hyphema Model Introduction[J]. ZHONGHUA YANGSHENG BAOJIAN, 2023, 41(10): 1-4.

参考文献

[1] 王凤云,陆晓和,张彩霞,等.兔眼前房积血并发高眼压时角膜的病理改变[J].南方医科大学学报,2010,30(3):565-568.
[2] 鲍旭辉. 多聚二磷酸腺苷核糖聚合酶在脑出血后血红蛋白所致脑水肿中作用的实验研究[D].上海:复旦大学,2008.
[3] CABRERA M P, CHIHUAILAF R H.Antioxidants and the integrity of ocular tissues[J]. Veterinary medicine international,2011,2011:905153.
[4] 杨红,刘淑芳,陈芳.依地酸二钠-几丁糖凝胶对兔角膜血染铁离子析出的作用[J].眼外伤职业眼病杂志,2010,32(4):246-250.
[5] 李宗信,黄小波,敖平,等.电离子导入技术国内外研究近况[J].中华理疗杂志,2000,23(6):372-374.
[6] 张志勇,孙延斌,杨金永.经皮离子导入技术给药的研究进展[J].牡丹江医学院学报,2016,37(4):119-122.
[7] GIRI T K, CHAKRABARTY S, GHOSH B.Transdermal reverse iontophoresis: A novel technique for therapeutic drug monitoring[J]. Journal of Controlled Release,2017,246:30-38.
[8] SUN S, DIAO H, ZHAO F, et al.Extraction of iron from the rabbit anterior chamber with reverse iontophoresis[J]. Journal of Ophthalmology,2015,2015:425438.
[9] WOLLENSAK G, MUCHAMEDJAROW F, FUNK R.Evaluation of Treatment by Pulsed Electromagnetic Fields in a Rabbit Hyphema Model[J]. Ophthalmologica,2003,217(2):143-147.
[10] 刘淑芳,杨红,陈芳.角膜血染实验动物模型建立及其病理改变[J].眼外伤职业眼病杂志,2006,28(9):641-644.
[11] FLIEGER J, DOLAR-SZCZASNY J, REJDAK R, et al.The Multi-Elemental Composition of the Aqueous Humor of Patients Undergoing Cataract Surgery, Suffering from Coexisting Diabetes, Hypertension, or Diabetic Retinopathy[J]. International journal of molecular sciences,2021,22(17):9413.
[12] 刘淑芳. 依地酸二钠—几丁糖眼用凝胶对角膜血染疗效的体外实验研究[D].武汉:华中科技大学,2006.
[13] 庞秀琴,何雷,王文伟,等.房水铁离子含量测定与眼铁质沉着症相关性的研究[J].眼科,2000,9(3):158-160.
[14] LQBAL Z, MUHAMMAD Z, SHAH M T, et al.Relationship between the concentration of copper and iron in the aqueous humour and intraocular pressure in rabbits treated with topical steroids[J]. Clin Exp Ophthalmol, 2002,30(1):28-35.

反向离子导入对兔眼前房积血治疗的实验研究

Evaluation of Treatment by Reverse Iontophoresis in a Rabbit Hyphema Model Introduction

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 6

编辑推荐

Metrics

本文评价

[1]	孟桂莲. 维生素D联合右旋糖酐铁治疗小儿贫血的临床效果[J]. 中华养生保健, 2023, 41(17): 194-196.
[2]	王栋. 血液检验红细胞参数鉴别诊断贫血的价值研究[J]. 中华养生保健, 2022, 40(8): 173-175.
[3]	朱文静. 血常规检验对缺铁性贫血与地中海贫血的鉴别价值[J]. 中华养生保健, 2021, 39(2): 166-168.
[4]	郭杰, 董慎山. 小儿缺铁性贫血患者应用蛋白琥珀酸铁口服溶液对提高患儿自身免疫功能的影响[J]. 中华养生保健, 2020, 38(4): 57-59.
[5]	刘淑芬. 营养干预对婴幼儿缺铁性贫血发生率的影响研究[J]. 中华养生保健, 2020, 38(3): 109-110.
[6]	刘树霞. 综合护理在小儿缺铁性贫血治疗中的作用分析[J]. 中华养生保健, 2020, 38(10): 75-76.