LAMP技术在重症肺炎患者病原体检测中的价值

摘要/Abstract

摘要： 目的探讨与分析环介导等温扩增（loop-mediatedisothermal amplification,LAMP）技术在重症肺炎患者病原体检测中的价值。方法选择2020年4月~2022年4月宁夏中西医结合医院收治的重症肺炎患者100例作为研究对象,所有患者都给予LAMP技术和传统痰培养检测,以传统痰培养检查作为判断的金标准,探讨LAMP检测对重症肺炎患者早期诊断的临床价值。结果在100例患者中,传统痰培养判断为单一病原体感染65例,合并病原体感染35例。在100例患者中,LAMP技术平均检测时间为（2.10±0.02）h,LAMP技术检出肺炎链球菌21例,金黄色葡萄球菌11例,耐甲氧西林金黄色葡萄球菌18例,大肠埃希菌15例,铜绿假单胞菌14例,鲍曼不动杆菌22例,肺炎克雷伯菌10例,嗜麦芽窄食单胞菌9例,其他22例。LAMP技术判断为单一病原体感染62例,合并病原体感染38例,LAMP技术在重症肺炎患者病原体合并感染的诊断敏感性与特异性分别为100.00%（35/35）和95.38%（62/65）。结论重症肺炎患者多伴随病原体感染情况,LAMP技术能准确快速检出重症肺炎患者病原体感染情况,具有特异性高等优势,有很好的临床应用价值。

关键词: 环介导等温扩增技术, 重症肺炎, 病原体, 传统痰培养

Abstract: Objective To explore and analysis the values of loop-mediated isothermal amplification (LAMP) technology in pathogen detection in patients with severe pneumonia. Methods From April 2020 to April 2022, 100 cases of patients with severe pneumonia who were treated in Ningxia Hospital of Integrated Traditional Chinese and Western Medicine were selected as the research subjects. All patients were given LAMP technology and traditional sputum culture test. Traditional sputum culture test was used as the gold standard, to explore the clinical values of LAMP detection in the early diagnosis of severe pneumonia patients. Results In the 100 patients, there were 65 cases were diagnosed as single pathogen infection by traditional sputum culture, and 35 cases were combined pathogen infection. In the 100 patients, the average detection time of LAMP technology was (2.10±0.02)h, LAMP technology detected 21 cases of Streptococcus pneumoniae, 11 cases of Staphylococcus aureus, and 18 cases of methicillin-resistant Staphylococcus aureus, 15 cases of Escherichia coli, 14 cases of Pseudomonas aeruginosa, 22 cases of Acinetobacter baumannii, 10 cases of Klebsiella pneumoniae, 9 cases of Stenotrophomonas maltophilia, and 22 other cases. LAMP technology judged 62 cases of single pathogen infection and 38 cases of combined pathogen infection. The sensitivity and specificity of LAMP technology in the diagnosis of pathogen combined infection in patients with severe pneumonia were 100.00% (35/35) and 95.38% (62/65), respectively. Conclusion Severe pneumonia patients are often accompanied by pathogen co-infection. LAMP technology can accurately and fast detect pathogen co-infection in patients with severe pneumonia. It has the advantages of short time and high specificity and has good clinical application value.

Key words: loop-mediated isothermal amplification technique, severe pneumonia, pathogen, traditional sputum culture

中图分类号:

R446.5

张薇, 任春平, 张文莉. LAMP技术在重症肺炎患者病原体检测中的价值[J]. 中华养生保健, 2022, 40(23): 1-4.

ZHANG Wei, REN Chun-ping, ZHANG Wen-li. The Value of LAMP Technology in Pathogen Detection in Patients with Severe Pneumonia[J]. ZHONGHUA YANGSHENG BAOJIAN, 2022, 40(23): 1-4.

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